Agdia, Inc. has added another test kit to their Tomato brown rugose fruit virus (ToBRFV) diagnostic suite. AmplifyRP® XRT for ToBRFV is the second of three ToBRFV diagnostic assays to be launched by Agdia in the first half of 2021. Their high-specificity ELISA assay for ToBRFV was released on January 7. An ImmunoStrip® lateral flow device for ToBRFV is expected to be released in the coming months (Q1 or Q2 of 2021.)
Agdia’s AmplifyRP XRT for ToBRFV has been validated for use with tomato and pepper seeds and leaf. As a rapid, field-deployable molecular method requiring far less training than traditional PCR methods, this assay provides users with greater flexibility to deploy detection capabilities where they need it, when they need it. Use cases for this assay include firstly laboratory-based molecular diagnosis with crude or purified extracts with faster time-to-result than traditional PCR or qPCR methods. This assay can be used with Agdia’s AmpliFire isothermal fluorometer or with most commonly-used real-time PCR machines.
Secondly, the new kit offers in-field monitoring at remote production sites as a stand-alone assay. Directly test ELISA extracts for molecular confirmation of serological screening results, allowing you to act immediately to address production issues.
Agdia’s AmplifyRP XRT for ToBRFV is highly specific to ToBRFV and has been experimentally proven to detect isolates from around the world. No cross-reactivity was observed with high titer samples from other Tobamoviruses, including Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), Pepper mild mottle virus (PMMoV), Tobacco mosaic virus (TMV), Tomato mosaic virus (ToMV), Tobacco mild green mosaic virus (TMGMV), Zucchini Green Mottle Mosaic Virus (ZGMMV) and more.
Tamatiebruin rugose-vrugte-virus is 'n weerstandbrekende Tobamovirus wat ernstige ekonomiese verliese in solanaceous gewasse veroorsaak, insluitend Solanum lycopersicum (tamatie) en Capsicum spp. (peper). Dit veroorsaak simptome tipies van Tobamovirusse, wat mosaïek en chlorose op die blare insluit, asook verkleuring en vervorming van die vrugte. Hierdie simptome verlaag die opbrengs en maak vrugte onbemarkbaar.
Tamatie- en pepersaad, oorplantings en vrugte uit sekere lande is onderhewig aan 'n USDA-APHIS Federal Import Order in die Verenigde State. Tamatiebruin rugose-vrugtevirus is ook deur EPPO (Europese en Mediterreense plantebeskermingsorganisasie) as 'n kwarantynpatogeen geklassifiseer.
Vir meer inligting:
Agdia Inc.
52642 County Road 1
Elkhart, IN 46514
www.agdia.com